Dynamics of keratin assembly: exogenous type I keratin rapidly associates with type II keratin in vivo
نویسندگان
چکیده
منابع مشابه
Dynamics of keratin assembly: exogenous type I keratin rapidly associates with type II keratin in vivo
Keratin intermediate filaments (IF) are obligate heteropolymers containing equal amounts of type I and type II keratin. We have previously shown that microinjected biotinylated type I keratin is rapidly incorporated into endogenous bundles of keratin IF (tonofilaments) of PtK2 cells. In this study we show that the earliest steps in the assembly of keratin subunits into tonofilaments involve the...
متن کاملThe genomic organization of type I keratin genes in mice.
We isolated two new keratin cDNAs by screening a cDNA library constructed from poly(A)+ RNA of the dorsal and abdominal skin of C57BL/10J mice with a probe of human KRT14. Due to its high sequence homology to human keratin 17 cDNA, one full-length cDNA is most likely to be mouse keratin 17 (Krt1-17) cDNA. The other is the putative full-length cDNA of a novel type I keratin gene, designated Krt1...
متن کاملSequence and expression of a human type II mesothelial keratin
Using mRNA from cultured human mesothelial cells, we constructed bacterial plasmids and lambda phage vectors that contained cDNA sequences specific for the keratins expressed in these cells. A cloned cDNA encoding keratin K7 (55 kD) was identified by positive hybrid selection. Southern Blot analysis indicated that this sequence is represented only once in the human genome, and Northern Blot ana...
متن کاملKeratin 13 is a more specific marker of conjunctival epithelium than keratin 19
Introduction To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. Methods: Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea wer...
متن کاملKeratin nanoparticles: synthesis and application for Cu(II) removal
A straightforward procedure to synthesize keratin nanoparticles (KNP) from chicken feathers was introduced. The characterization of the synthesized nanoparticles was done using Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD) patterns and transmission electron microscopy (TEM). The FTIR analysis revealed no significant chemical change after...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1993
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.122.1.123